psychiatryПСИХИАТРИЯPsychiatry (Moscow) (Psikhiatriya)1683-83192618-6667FSBSI “The Mental Health Research Centre”; LLC «Publisher «MIA»10.30629/2618-6667-2018-79-69-78psychiatry-371Research ArticleВОПРОСЫ ПСИХОПАТОЛОГИИ, КЛИНИЧЕСКОЙ И БИОЛОГИЧЕСКОЙ ПСИХИАТРИИУльтраструктурные нарушения отростков астроцитов в префронтальной и зрительной коре при шизофренииUltrastructural abnormalities of astrocytic cell processes in prefrontal and visual cortex in schizophreniaВихреваО. В.VikhrevaO. V.

Вихрева Ольга Васильевна - кандидат биологических наук, старший научный сотрудник лаборатории клинической нейроморфологии.

Москва.

Olga V. Vikhreva - Cand. of Sci. (Biol.), senior researcher, laboratory of clinical neuromorphology.

Moscow.

vikhreva@mail.ruРахмановаВ. И.RakhmanovaV. I.

Рахманова Валентина Ивановна - ведущий программист, отдел исследования мозга, лаборатория ультраструктуры и цитохимии мозга.

Москва.

Valentina I. Rakhmanova - application programmer, department of brain research, laboratory of the ultrastructure and cytochemistry/

Moscow.

val_ivan@list.ruКлинцоваА. Ю.KlintsovaA. Ju.

Клинцова Анна Юрьевна - кандидат биологических наук, профессор факультета психологии и изучения мозга, Университет штата Делавер.

klintsova@psych.udel.eduГринауУ. Т.GreenoughW. T.

Гринау Уильям Т. - профессор. 

William T. Greenough - PhD, swanland professor, Beckman Institute.

УрановаН. А.UranovaN. A.

Уранова Наталия Александровна - доктор медицинских наук, заведующая лабораторией клинической нейроморфологии.

Москва.

Natalya A. Uranova - Dr. of Sci. (Med.), head of the laboratory of clinical neuromorphology.

Moscow.

uranovan@mail.ruНаучный центр психического здоровьяРоссияMental Health Research CentreRussian FederationУниверситет штата Иллиноис; Университет штата ДелаверСоединённые Штаты АмерикиUniversity of Illinois at Urbana-Champaign; University of DelawareUnited StatesУниверситет штата ИллиноисСоединённые Штаты АмерикиUniversity of Illinois at Urbana-ChampaignUnited States2018250420190796978Copyright © Вихрева О.В., Рахманова В.И., Клинцова А.Ю., Гринау У.Т., Уранова Н.А., 20192019Вихрева О.В., Рахманова В.И., Клинцова А.Ю., Гринау У.Т., Уранова Н.А.Vikhreva O.V., Rakhmanova V.I., Klintsova A.J., Greenough W.T., Uranova N.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.journalpsychiatry.com/jour/article/view/371Цель

Цель: провести морфометрическое электронномикроскопическое исследование отростков астроцитов (0А) в префронтальной коре (ПФК) и зрительной коре (ЗК), включая 0А, прилежащие к синапсам на шипиках дендритов, в ПФК и ЗК при шизофрении и в контроле.

Материал и методы

Материал и методы: исследованы ПФК (поле 10 по Бродману) и ЗК (поле 17 по Бродману) в 20 аутопсийных случаях шизофрении и 16 нормальных контрольных случаях. Определяли объемную фракцию (Vv) 0А и содержащихся в них митохондрий в слоях I и II в группах шизофрении и контрольной.

Результаты

Результаты: в ПФК в слое I показано снижение Vv 0А (-31%, р < 0,05), содержащихся в них митохондрий (-48%, р < 0,05) и Vv 0А, прилежащих к аксошипиковым синапсам (-44%, р < 0,05) при шизофрении по сравнению с контролем. В ЗК в слое I значимых изменений не найдено. В слое II только в ЗК показано снижение Vv 0А (-38%, р < 0,01).

Заключение

Заключение: регрессивное снижение объемной фракции 0А при шизофрении регионально специфично, может быть связано со снижением численной плотности аксошипиковых синапсов и нарушением функциональной активности в ПФК. Эти изменения могут быть результатом дизонтогенеза при шизофрении.

Aim

Aim: to perform electron microscopic morphometric study of astrocytic processes (AP), including those directly adjacent to axospinous synapses, in the prefrontal cortex (PFC) and visual cortex (VC) in postmortem brains of schizophrenia patients and normal controls.

Material and methods

Material and methods: PFC and VC (Brodmann areas 10 and 17) obtained from autopsies of 20 cases of schizophrenia and 16 normal controls. Volume fraction (Vv) of APand Vv of mitochondria inside these processes, including those adjacentto axo-spinous synapses, were estimated layers I and II of the PFC and VC in the schizophrenia and control groups.

Results

Results: in layer I of the PFC, a significant decrease in Vv of AP (-31%, p < 0,05), of AP mitochondria (-48%, p < 0,05) and Vv of AP adjacent to axospinous synapses (-44%, p < 0,05) were found in schizophrenia as compared to controls. No significant changes in any of those parameters were detected in the VC of the schizophrenia group compared with control group. In layer II a significantly lower Vv of AP were found only in the VC in schizophrenia (-38%, p < 0,01).

Conclusion

Conclusion: regressive decrease in the Vv of AP in schizophrenia is region-specific and might be associated with the decrease in the density and the dysfunction of axospinous synapses in the PFC. The changes might be of development origin.

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The authors declare that there are no conflicts of interest present.