Мoзговая изоформа креатинкиназы в лимбической коре мозга в норме и при шизофрении

Aim. To compare levels of brain isoform creatine phosphokinase (CPK BB) in autopsied anterior and posterior cingulate cortex from patients with schizophrenia and control subjects.Methods. Extracts of readily soluble and membrane-associated proteins were prepared from autopsied samples of anterior (Brodmann area 24) and posterior (Brodmann area 23) cingulate cortex from patients with schizophrenia ( n = 15) and from control group ( n = 14), the samples were matched by age and postmortem interval. CPK enzymatic activity was measured by determination of nonorganic phosphate, amounts of immunoreactive CPK В were estimated by ECL-Western blotting using monoclonal antibodies.Results. Significant decrease in CPK activity and amounts of immunoreactive CPK В was observed in fractions of readily soluble proteins in cingulate cortex of patients with schizophrenia compared with controls ( p < 0,01). No significant difference in CPK activity and amount of CPK B was found in membrane-associated protein fractions from cingulate cortex (areas 23 and 24) of patients with schizophrenia and controls.Conclusion. Significantly reduced level of the central energy metabolism enzyme (brain CPK BB) indicates the significant impairment of energy metabolism in cingulate cortex of patients with schizophrenia and represents important aspect of schizophrenia pathogenesis.

шизофрения, мозг человека, креатинфосфокиназа ВВ, моноклональные антитела, ECL-вестерн-иммуноблоттинг, schizophrenia, human brain, creatine phosphokinase BB, monoclonal antibodies, ECL-Western blotting, schizophrenia, human brain, creatine phosphokinase BB, monoclonal antibodies, ECL-Western blotting

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