Снижение количества фосфатактивируемой глутаминазы и глутаматдекарбоксилазы в мозге при болезни Альцгеймера

The aim of study. To present results of original comparative study on levels of phosphate-activated glutaminase (PAG) and glutamic acid decarboxylase isoenzymes (GAD65/67) and brain isoform creatine kinase (CK BB) in autopsied cerebellum samples from AD patients and matched controls (13 cases in each group).Material and methods. Samples obtained after autopsy were frozen and stored at -80 °C. The groups are matched by sex, age, postmortem interval and cause of death. Protein extracts from cerebellum tissues were obtained after removing of nuclei and cell debris by centrifugation and treatment of the obtained fractions with detergent (SDS). Relative amounts of these enzymes were determined using SDS-PAAG-electrophoresis with the following semi-quantitative ECL-Western-immunoblotting with chemiluminescence detection.Results. The majority of samples from the AD group contained less amount of PAG in comparison with control samples, the Mann Whitney U-test demonstrated a significant between-group difference ( p = 0,004). Differences between groups in GADlevels were also statistically significant: for GAD67 p = 0,00004, for GDK65 p = 0,0002. Significant correlation between the levels of the both enzymes was not observed in the control group such as in AD group. As for CK BB it was also decreased in AD compared to controls ( p = 0,0023).Conclusions. Decreased levels of these enzymes in AD cerebellum suggest significantly impaired glutamate, GABA and energy metabolism in this brain region, which apparently involved in AD pathogenesis.

фосфатактивированная глутаминаза, глутаматдекарбоксилаза, креатинкиназа, мозг, болезнь Альцгеймера, phosphate-activated glutaminase, glutamate decarboxylase, creatine kinase, brain, Alzheimer’s disease

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